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产品描述：鸡白介素4(IL-4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL-4含量。IL-4是重要的免疫调节细胞因子，在多种免疫和炎症反应中起关键作用。研究发现它可促进B细胞增殖分化，调节Th2细胞免疫，与哮喘、特应性皮炎等疾病的发生发展密切相关。目前围绕它阻断信号传导等机制的研究，有望带来新治疗策略。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于科研领域对鸡免疫系统功能的研究，如传染病模型中的细胞因子动态分析、疫苗免疫效果评估或免疫相关疾病的分子机制探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IL-4
Uniprot No.：Q5W4U1
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 80-95 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 88-101 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 86-106 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 82-100 | |||||
回收率：
| The recovery of chicken IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-97 | ||||
| EDTA plasma (n=4) | 92 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected8001.7261.8251.7761.6654001.3361.3831.3601.2492001.0600.9811.0210.9101000.7140.7060.7100.599500.4810.4750.4780.367250.3070.2920.3000.18912.50.2270.2140.2210.11000.1080.1130.111 | ||||||
货期：3-5 working days
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