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产品描述：豚鼠白介素4(IL-4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL-4含量。IL-4是一种多效细胞因子，参与免疫应答的调节。主要由肥大细胞、T细胞等分泌，通过IL-4 RA受体招募γc链，形成I型信号复合物，介导细胞生长、抗凋亡、基因激活和分化。此外，IL-4还参与调节T细胞分化、免疫球蛋白类转换等，在免疫和炎症过程中发挥重要作用。试剂盒检测范围为3.12 pg/mL-200pg/mL，适用于豚鼠模型的免疫机制研究，特别是在过敏性疾病、寄生虫感染、哮喘模型等Th2型免疫反应相关科研领域，为评估实验动物体内IL - 4动态变化提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IL-4
Uniprot No.：G0WM66
种属：Guinea Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 93-105 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-102 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 97-107 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of guinea pig IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.3752.2942.3352.1601001.9031.8021.8531.678501.3551.3761.3661.191250.9030.9140.9090.73412.50.6500.6410.6460.4716.250.4230.4100.4170.2423.120.3170.3120.3150.14000.1760.1730.175 | ||||||
货期：3-5 working days
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