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产品描述：豚鼠白介素5(IL-5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL5含量。IL5是重要的治疗靶点。它主要由活化的T淋巴细胞产生，能促进嗜酸性粒细胞的生长、分化和存活。针对IL5的研究主要围绕阻断其与受体结合，以减少嗜酸性粒细胞数量，进而治疗哮喘、嗜酸性粒细胞增多症等嗜酸性粒细胞相关疾病。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于豚鼠疾病模型的免疫机制研究，例如哮喘、寄生虫感染等实验中的炎症因子动态监测，或用于评估免疫调节药物对IL - 5通路的影响。组织匀浆样本可用于分析肺、骨髓等靶器官的局部IL - 5表达水平，为科研人员提供稳定可靠的定量数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL5Interleukin-5 ELISA Kit; IL-5 ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
缩写：IL5
Uniprot No.：O08987
种属：Guinea Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 89-99 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 98-108 | |||||
| 1:8 | Average % | 100 | ||||
| Range % | 91-105 | |||||
回收率：
| The recovery of guinea pig IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 92-97 | ||||
| EDTA plasma (n=4) | 92 | 84-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.5162.4892.5032.39110001.3841.3561.3701.2585000.7330.6980.7160.6042500.3840.3750.3800.2681250.2530.2340.2440.13262.50.1870.1810.1840.07231.250.1420.1330.1380.02600.1130.1110.112 | ||||||
货期：3-5 working days
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