首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠L氨基酸氧化酶(IL4I1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的IL4I1含量。IL4I1靶点在相关疾病研究中备受关注。它具有独特的生物学背景，在免疫调节等生理过程里发挥作用。研究机制方面，多聚焦于其参与的信号通路以及与疾病发生发展的关联，为开发针对性治疗手段、攻克疑难病症提供理论依据。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，科研人员可将其应用于免疫学基础研究、肿瘤模型构建、炎症性疾病机制探索等领域，例如评估不同病理状态下IL4I1在血液或组织中的表达水平，或通过体外细胞实验分析其功能调控网络，为揭示IL4I1在免疫代谢中的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il4i1 ELISA Kit; Fig1 ELISA Kit; L-amino-acid oxidase ELISA Kit; LAAO ELISA Kit; LAO ELISA Kit; EC 1.4.3.2 ELISA Kit; Interleukin-4-induced protein 1 ELISA Kit; IL4-induced protein 1 ELISA Kit; Protein Fig-1 ELISA Kit; mFIG1 ELISA Kit
缩写：IL4I1
Uniprot No.：O09046
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL4I1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 101 | |||||
| Range % | 92-105 | ||||||
| 1:2 | Average % | 94 | |||||
| Range % | 90-99 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 87-98 | ||||||
| 1:8 | Average % | 92 | |||||
| Range % | 85-95 | ||||||
回收率：
| The recovery of mouse IL4I1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 103 | 94-107 | |||||
| EDTA plasma (n=4) | 98 | 90-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10001.7801.8141.7971.5965001.3161.3011.3091.1082500.9540.9390.9470.7461250.7160.7320.7240.52362.50.4880.4980.4930.29231.20.4060.4000.4030.20215.60.3060.2940.3000.09900.2030.1990.201 | |||||||
货期：3-5 working days
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