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产品描述：鸡白介素6(IL-6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL6含量。IL6是一种多功能细胞因子，由多种细胞产生，参与免疫、炎症和代谢等生理过程。其研究机制涉及与IL-6R结合，激活JAK/STAT3等信号通路，调节细胞生长、分化和存活。IL6在多种疾病如炎症、癌症和自身免疫性疾病中发挥重要作用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，其动态监测对家禽免疫机制研究、疫苗效力评估及感染性疾病模型构建具有重要意义。适用于科研实验中定量分析鸡类样本的IL - 6表达水平，可为家禽免疫学基础研究、病原体感染机制探索及抗炎药物开发提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL6 ELISA Kit; Interleukin-6 ELISA Kit; ChIL-6 ELISA Kit; IL-6 ELISA Kit
缩写：IL6
Uniprot No.：Q90YI0
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 85-97 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 85-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-100 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 85-100 | |||||
回收率：
| The recovery of chicken IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 91-105 | ||||
| EDTA plasma (n=4) | 96 | 88-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.4872.5702.5292.454 5002.2042.2302.2172.142 2501.8001.7641.7821.707 1251.3901.3941.3921.317 62.50.9170.9500.9340.859 31.20.5840.5680.5760.501 15.60.3480.3620.3550.280 00.0740.0750.075 | ||||||
货期：3-5 working days
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