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A micro ELISA plate --- The 96-well plate has been pre-coated with anti-rat IL-6 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --- Dilute a bottle of standard at dilution series, read the OD values, and then draw a standard curve.
Biotin-labeled IL-6 antibody (100 x concentrate) 1 x 120 μl ---Act as the detector antibody.
HRP-avidin (100 x concentrate) 1 x 120 μl --- Avidin binds to the detection antibody, and HRP catalyzes the TMB substrate and thus changes the solution color.
Biotin-antibody Diluent 1 x 15 ml ---Dilute the Biotin-antibody solution.
HRP-avidin Diluent 1 x 15 ml ---Dilute the HRP-avidin solution.
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
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