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产品描述：绵羊白介素6(IL-6)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL6含量。IL6是一个重要靶点。它是一种多功能细胞因子，参与免疫调节、急性期反应等生理过程。其异常表达与多种疾病如炎症性疾病、癌症等相关。研究聚焦于其信号传导通路，旨在开发针对IL6的药物来阻断异常信号，以治疗相关疾病。试剂盒检测范围为2 pg/mL-80 pg/mL，适用于科研领域中对绵羊炎症性疾病模型的机制研究、免疫调节药物开发评估以及畜牧兽医研究中宿主免疫状态监测，为探索IL - 6在先天免疫和适应性免疫中的信号通路调控提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL6 ELISA Kit; Interleukin-6 ELISA Kit; IL-6 ELISA Kit
缩写：IL6
Uniprot No.：P29455
种属：Ovis aries (Sheep)
样本类型：serum, plasma, tissue homogenates
检测范围：2 pg/mL-80 pg/mL
灵敏度：2 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 90-98 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-99 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-101 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 88-98 | |||||
回收率：
| The recovery of sheep IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 90-103 | ||||
| EDTA plasma (n=4) | 95 | 89-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 02.5412.4392.490 21.3821.3521.367 60.7560.7850.771 18.40.4050.4120.409 400.2190.2240.222 800.1260.1230.125 | ||||||
货期：3-5 working days
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