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产品描述：鱼白介素6(IL-6)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL-6含量。IL-6是重要的炎症细胞因子，在免疫调节、造血等生理过程中发挥关键作用。异常升高时与多种疾病如自身免疫病、肿瘤等的发生发展密切相关。其研究机制主要聚焦于阻断IL-6与其受体结合，以抑制下游信号通路从而达到治疗目的。试剂盒检测范围为12.5 pg/mL-200 pg/mL，本试剂盒适用于水产养殖中鱼类健康监测、病原感染模型建立、免疫增强剂效果评估等科研场景，亦可用于比较医学中脊椎动物免疫系统的进化机制探究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：il6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
缩写：IL-6
Uniprot No.：A0S0B0
种属：Fish
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 pg/mL-200 pg/mL
灵敏度：12.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 82-94 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 94-107 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 90-101 | |||||
回收率：
| The recovery of fish IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 91-104 | ||||
| EDTA plasma (n=4) | 91 | 84-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 2000.1280.1330.131 1000.2690.2580.264 500.4490.4310.440 250.8510.8420.847 12.51.8131.7831.798 | ||||||
货期：3-5 working days
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