首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：仓鼠白介素6(IL-6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL-6含量。IL-6是重要细胞因子，在免疫调节、炎症反应等生理病理过程中起关键作用。其过度表达与多种疾病如类风湿关节炎、新冠肺炎等密切相关。研究机制主要围绕抑制IL-6及其受体信号通路，以调控炎症反应和细胞增殖等，为疾病治疗提供方向。试剂盒检测范围为7.8 pg/mL-500 pg/mL，科研人员可将其应用于炎症机制研究、免疫治疗模型评估或药物研发中的细胞因子动态监测，特别适合体外实验环境下的定量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IL-6
种属：Mesocricetus auratus (Golden hamster)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of hamster IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-92 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 91-100 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of hamster IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 94-104 | ||||
| EDTA plasma (n=4) | 91 | 85-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.5762.4562.5162.3512502.1072.0762.0921.9271251.4231.4171.4201.25562.50.7300.6790.7050.54031.20.4570.4700.4640.29915.60.3260.3080.3170.1527.80.2620.2470.2550.09000.1610.1680.165 | ||||||
货期：3-5 working days
×
