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产品描述：马白介素6(IL-6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL-6含量。IL-6是一个重要靶点。它是一种多功能细胞因子，在免疫调节、炎症反应等过程中发挥关键作用。异常的IL-6表达与多种疾病如自身免疫病、癌症等相关。研究主要围绕抑制其活性或阻断其信号传导通路，以探索疾病治疗新策略。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于体外培养体系、疾病模型或生物标志物研究中的IL - 6动态监测；支持科研人员开展炎症机制探索、药物干预效果评估或病理生理学研究，为生命科学基础研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
缩写：IL-6
Uniprot No.：Q95181
种属：Equus caballus (Horse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of horse IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 84-98 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-101 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 96-108 | |||||
回收率：
| The recovery of horse IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-97 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.2672.2352.2512.1361001.4821.5891.5361.421500.9540.9990.9770.862250.6020.6120.6070.49212.50.4320.4520.4420.3276.250.2690.2890.2790.1643.120.2180.2300.2240.10900.1110.1190.115 | ||||||
货期：3-5 working days
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