首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠白介素6受体(IL-6R)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL6R含量。IL6R即白细胞介素 6 受体，在人体免疫调节等生理过程中起关键作用。其异常与多种炎症性疾病、自身免疫病及肿瘤发生发展相关。研究通过阻断 IL6R 信号传导，抑制相关细胞因子释放，为相关疾病治疗提供新思路。试剂盒检测范围为0.625 ng/mL-40 ng/mL，该产品适用于基础科研中炎症性疾病模型评估、免疫调控机制研究或药物干预效果分析等领域，为科研工作者探索IL - 6/IL - 6R信号通路提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il6r; Il6ra; Interleukin-6 receptor subunit alpha; IL-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; IL-6R 1; CD antigen CD126
缩写：IL6R
Uniprot No.：P22273
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-6R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 99 | ||||
| Range % | 91-103 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 95-104 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 83-92 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 84-97 | |||||
回收率：
| The recovery of rat IL-6R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 101 | 92-105 | ||||
| EDTA plasma (n=4) | 99 | 91-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.4922.4372.4652.289202.0771.9812.0291.853101.2891.3001.2951.11950.7990.7870.7930.6172.50.4700.4610.4660.2901.250.3940.3880.3910.2150.6250.2600.2520.2560.08000.1780.1740.176 | ||||||
货期：3-5 working days
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