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产品描述：鸡白介素8(IL-8)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CXCL8含量。CXCL8即趋化因子配体8，是重要的促炎细胞因子。它在炎症反应、肿瘤发生发展等过程中扮演关键角色。其研究机制主要围绕与受体结合，激活下游信号通路，促进细胞趋化、增殖等，在免疫调节和疾病病理进程研究中备受关注。试剂盒检测范围为1.56 pg/mL-400 pg/mL，该产品广泛用于家禽免疫机制研究、疫苗效力评估、病原感染模型建立等科研领域，特别适用于鸡传染性支气管炎、新城疫等疾病模型中炎症因子的动态监测，以及免疫调节剂或饲料添加剂对免疫系统影响的量化评估。实验支持多种样本类型检测，组织匀浆检测功能为深入解析局部组织免疫反应提供技术保障。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CXCL8 ELISA Kit; CEF4 ELISA Kit; EMF1 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; 9E3 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; CEF-4 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Embryo fibroblast protein 1 ELISA Kit; EMF-1 ELISA Kit
缩写：CXCL8
Uniprot No.：P08317
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-400 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 102 | ||||
| Range % | 97-106 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 84-93 | |||||
| 1:4 | Average % | 104 | ||||
| Range % | 99-109 | |||||
| 1:8 | Average % | 103 | ||||
| Range % | 99-106 | |||||
回收率：
| The recovery of chicken IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-102 | ||||
| EDTA plasma (n=4) | 91 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 4000.1870.1830.185 1000.2590.2670.263 250.4710.4520.462 6.250.8620.8430.853 1.561.4101.3981.404 02.2512.1592.205 | ||||||
货期：3-5 working days
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