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产品描述：豚鼠白介素8(IL-8)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CXCL8含量。CXCL8（C-X-C motif chemokine ligand 8）是一种趋化因子，由巨噬细胞和其他细胞产生，具有促进血管生成和调节炎症反应的功能。其研究机制涉及对中性粒细胞的召集和脱颗粒，以及与多种疾病如癌症、炎症和肥胖的关联。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，广泛应用于豚鼠模型的感染性疾病机制研究、免疫调节实验及药物开发中的炎症因子动态监测，特别适用于呼吸道感染、皮肤炎症或肠道疾病等动物模型的病理分析，为科研人员提供稳定可靠的IL - 8浓度检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CXCL8 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Neutrophil attractant protein 1 ELISA Kit; NAP-1 ELISA Kit
缩写：CXCL8
Uniprot No.：P49113
种属：Guinea Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 85-100 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 88-102 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 90-104 | |||||
回收率：
| The recovery of guinea pig IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 95-102 | ||||
| EDTA plasma (n=4) | 94 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.2532.2452.2492.13410001.6311.6961.6641.5495001.1111.1351.1231.0082500.7170.7520.7350.6201250.4340.4230.4290.31462.50.3430.3540.3490.23431.250.2280.2410.2350.12000.1160.1130.115 | ||||||
货期：3-5 working days
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