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产品描述:人抑制素B(INH-B)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的INH-B含量。INH-B(抑制素B)是一种由卵巢和睾丸产生的蛋白质激素,属于TGF-β超家族。它在女性中主要由卵巢颗粒细胞产生,在男性中主要由睾丸支持细胞合成。INH-B通过负反馈机制抑制垂体FSH的分泌,参与调节生殖功能和代谢过程。近年来,INH-B在肥胖、糖尿病等代谢性疾病的研究中备受关注。试剂盒检测范围为4 pg/mL-800 pg/mL,生殖系统发育、性腺功能及生育能力评估等研究;生殖内分泌研究、生殖疾病机制探索(如多囊卵巢综合征、睾丸功能障碍等)以及动物模型评估;科研场景中不同样本类型的横向比对分析;生殖健康相关基础研究本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:N/A
缩写:INH-B
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:4 pg/mL-800 pg/mL
灵敏度:2 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Signal Transduction
测定原理:quantitative
测定方法:Competitive
精密度:
Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human INH-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 98 | ||||
Range % | 94-102 | |||||
1:2 | Average % | 93 | ||||
Range % | 89-97 | |||||
1:4 | Average % | 99 | ||||
Range % | 96-101 | |||||
1:8 | Average % | 92 | ||||
Range % | 86-96 |
回收率:
The recovery of human INH-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 84 | 80-88 | ||||
EDTA plasma (n=4) | 96 | 92-102 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
pg/mlOD1OD2Average 8000.2230.2240.224 2000.3550.3620.359 600.5560.5680.562 160.8170.8030.810 41.3741.2751.325 02.5082.4042.456 |
货期:3-5 working days
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