首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠抑制素B(INH-B)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的INH-B含量。试剂盒检测范围为1 pg/mL-200 pg/mL，本试剂盒通过高特异性抗体与样本中INH - B的竞争性结合反应实现定量分析，可广泛应用于生殖生物学研究、激素调控机制探索，以及生殖相关疾病动物模型（如睾丸功能障碍、多囊卵巢综合征等）的科研分析。其检测体系经过严格优化，能够稳定检测低浓度样本，为科研人员提供可靠的数据支持，适用于实验室中对大鼠模型生殖内分泌状态的基础研究与病理机制评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：INH-B
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1 pg/mL-200 pg/mL
灵敏度：0.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat INH-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-106 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-99 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of rat INH-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 87-104 | ||||
| EDTA plasma (n=4) | 97 | 91-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 2000.2400.2460.243 500.2910.3050.298 160.4210.4320.427 40.7220.6970.710 11.1171.1461.132 02.1882.1642.176 | ||||||
货期：3-5 working days
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