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产品描述：牛抑制素-βA链(INHBA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的INHBA含量。INHBA是一种靶点。它在细胞生长、分化等生理过程中或发挥重要作用。研究机制上，聚焦于其在信号通路的作用，探索其与疾病发生发展关联。深入了解它，有助于开发针对该靶点的药物，为相关疾病治疗带来新思路。试剂盒检测范围为15 pg/mL-600 pg/mL，适用于畜牧业中繁殖性能评估、生殖系统疾病模型研究，以及实验室中对INHBA在组织表达水平、激素调控网络的相关机制探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：INHBAInhibin beta A chain ELISA Kit; Activin beta-A chain ELISA Kit
缩写：INHBA
Uniprot No.：P07995
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：15 pg/mL-600 pg/mL
灵敏度：15 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine INHBA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 86-103 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 91-102 | |||||
回收率：
| The recovery of bovine INHBA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-100 | ||||
| EDTA plasma (n=4) | 96 | 91-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 6000.1610.1590.160 3000.3710.3530.362 1380.5860.5550.571 450.8670.8740.871 151.4891.4921.491 02.3682.4412.405 | ||||||
货期：3-5 working days
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