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产品描述：猪主要急性期蛋白( MAP)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ITIH4含量。试剂盒检测范围为37.5 ng/mL-2400 ng/mL，该产品适用于动物免疫学研究、疾病模型建立后的病理分析，以及抗炎药物或疫苗效果评估等科研场景，尤其可为猪的急性炎症反应机制研究、组织损伤修复实验提供定量检测支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ITIH4 ELISA Kit; IHRP ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H4 ELISA Kit; ITI heavy chain H4 ELISA Kit; ITI-HC4 ELISA Kit; Inter-alpha-inhibitor heavy chain 4 ELISA Kit; Inter-alpha-trypsin inhibitor family heavy chain-related protein ELISA Kit; IHRP ELISA Kit; Major acute phase protein ELISA Kit; MAP ELISA Kit
缩写：ITIH4
Uniprot No.：P79263
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：37.5 ng/mL-2400 ng/mL
灵敏度：9.38 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig MAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 92-104 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| 1:1600 | Average % | 86 | ||||
| Range % | 82-92 | |||||
回收率：
| The recovery of pig MAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-101 | ||||
| EDTA plasma (n=4) | 97 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 24000.1380.1410.140 12000.4570.4600.459 6000.9030.8980.901 3001.1341.1211.128 1501.5181.4831.501 751.6891.7061.698 37.52.0322.0492.041 02.1872.1142.151 | ||||||
货期：3-5 working days
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