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Mouse Beta-klotho(KLB) ELISA kit

  • 中文名: 小鼠β-klotho(KLB)ELISA kit
货号: IPD-EL012378MO
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    小鼠β-klotho(KLB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的KLB含量。KLB(β-Klotho)是一种跨膜蛋白,主要在肝脏和脂肪组织中表达,与FGF19结合后成为FGFR1c和FGFR4的高亲和力受体,调节胆汁酸合成和肝细胞增殖。KLB在多种代谢过程中发挥调节作用,其变异与NAFLD儿童肝损害有关,可能对肝细胞的脂毒性和炎症起保护作用。研究机制涉及FGF19-KLB-FGFRs信号通路,对理解相关疾病的发生发展具有重要意义。试剂盒检测范围为2.74 pg/mL-2000 pg/mL,可应用于代谢调控机制研究、基因敲除/过表达模型中KLB蛋白表达验证,以及药物干预或营养状态改变后体内KLB水平动态监测等科研场景。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Klb ELISA Kit; BetaklBeta-klotho ELISA Kit; BKL ELISA Kit; BetaKlotho ELISA Kit; Klotho beta-like protein ELISA Kit
  • 缩写:
    KLB
  • Uniprot No.:
    Q99N32
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    2.74 pg/mL-2000 pg/mL
  • 灵敏度:
    2.74 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Metabolism
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse KLB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    86


    Range %

    80-95


    1:2

    Average %

    97


    Range %

    91-102


    1:4

    Average %

    96


    Range %

    92-100


    1:8

    Average %

    91


    Range %

    86-98


  • 回收率:

    The recovery of mouse KLB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    92

    88-98


    EDTA plasma (n=4)

    97

    91-105


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    2000

    1.852

    1.956

    1.904

    1.740


    666.67

    1.592

    1.610

    1.601

    1.437


    222.22

    1.311

    1.298

    1.305

    1.141


    74.04

    0.955

    0.943

    0.949

    0.785


    24.69

    0.726

    0.755

    0.741

    0.577


    8.23

    0.573

    0.584

    0.579

    0.415


    2.74

    0.375

    0.374

    0.375

    0.211


    0

    0.162

    0.165

    0.164




  • 货期:
    3-5 working days

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