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产品描述：人层粘连蛋白亚基γ-1(LAMC1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LAMC1含量。试剂盒检测范围为0.78 ng/mL-50 ng/mL，适用于基础研究中LAMC1蛋白表达的定量评估，例如肿瘤微环境分析、组织修复机制研究或纤维化疾病模型中基质重塑的生物标志物探索，为细胞外基质相关科研提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LAMB2 ELISA Kit; lamc1 ELISA Kit; LAMC1_HUMAN ELISA Kit; Laminin B2 chain ELISA Kit; Laminin subunit gamma-1 ELISA Kit; Laminin, gamma 1 (formerly LAMB2) ELISA Kit; Laminin-1 subunit gamma ELISA Kit; Laminin-10 subunit gamma ELISA Kit; Laminin-11 subunit gamma ELISA Kit; Laminin-2 subunit gamma ELISA Kit; Laminin-3 subunit gamma ELISA Kit; Laminin-4 subunit gamma ELISA Kit; Laminin-6 subunit gamma ELISA Kit; Laminin-7 subunit gamma ELISA Kit; Laminin-8 subunit gamma ELISA Kit; Laminin-9 subunit gamma ELISA Kit; MGC87297 ELISA Kit; S-LAM gamma ELISA Kit; S-laminin subunit gamma ELISA Kit
缩写：LAMC1
Uniprot No.：P11047
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LAMC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 102 | |||||
| Range % | 98-110 | ||||||
| 1:2 | Average % | 93 | |||||
| Range % | 89-97 | ||||||
| 1:4 | Average % | 88 | |||||
| Range % | 84-92 | ||||||
| 1:8 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
回收率：
| The recovery of human LAMC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 91 | 85-95 | |||||
| EDTA plasma (n=4) | 95 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected502.0122.1482.0801.888251.7011.7931.7471.55512.51.4121.3321.3721.1806.250.9010.9450.9230.7313.120.6330.6010.6170.4251.560.4120.4380.4250.2330.780.3050.3140.3100.11800.1940.1890.192 | |||||||
货期：3-5 working days
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