首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人苗条素受体(LR/Ob-R)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LEPR含量。LEPR即瘦素受体，其背景在于它是瘦素发挥作用的关键分子。研究机制方面，瘦素与LEPR结合后，激活细胞内信号传导途径，调节下丘脑神经元活动，影响食欲、能量代谢等，在肥胖、代谢相关疾病的发生发展中扮演重要角色。试剂盒检测范围为3.12 pg/mL-200 pg/mL，本试剂盒可高效应用于肥胖机制研究、代谢综合征模型构建、糖尿病相关信号通路探索等科研领域。还可用于体外研究瘦素受体表达调控、药物干预效果评估或疾病生物标志物筛选。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD 295 ELISA Kit; CD295 ELISA Kit; CD295 antigen ELISA Kit; Db ELISA Kit; Fa ELISA Kit; HuB219 ELISA Kit; LEP R ELISA Kit; LEP-R ELISA Kit; LEPR ELISA Kit; LEPR_HUMAN ELISA Kit; LEPRD ELISA Kit; Leptin receptor ELISA Kit; Leptin receptor fatty ELISA Kit; Leptin receptor gene related protein ELISA Kit; Leptin receptor precursor ELISA Kit; Leptin receptor precursor ELISA Kit; OB R gene related protein ELISA Kit; OB receptor ELISA Kit; OB-R ELISA Kit; OB-RGRP ELISA Kit; obl ELISA Kit; Obr ELISA Kit
缩写：LEPR
Uniprot No.：P48357
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LR/Ob-R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 90 | ||||
| Range % | 86-93 | |||||
| 1:400 | Average % | 100 | ||||
| Range % | 97-103 | |||||
| 1:800 | Average % | 94 | ||||
| Range % | 89-97 | |||||
| 1:1600 | Average % | 98 | ||||
| Range % | 94-101 | |||||
回收率：
| The recovery of human LR/Ob-R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 91-97 | ||||
| EDTA plasma (n=4) | 97 | 92-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.6002.5842.5922.4691002.3192.2222.2712.148501.7251.7111.7181.595251.0991.0681.0840.96112.50.6280.6440.6360.5136.250.3820.3930.3880.2653.120.2430.2570.2500.12700.1220.1240.123 | ||||||
货期：3-5 working days
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