首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠髓磷脂碱性蛋白(MBP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MBP含量。髓鞘碱性蛋白（MBP）是一种在中枢神经系统（CNS）髓鞘化过程中至关重要的蛋白质。其研究机制涉及MBP与金属酶的相互作用，以及作为药物设计的靶点。通过理解MBP的结构和功能，研究人员旨在开发新的治疗方法，以诊断和治疗与MBP相关的疾病，如多发性硬化症。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于基础医学研究中MBP表达调控机制探索，如多发性硬化症模型、创伤性脑损伤评估或神经再生相关实验，也可用于药物研发过程中神经保护效果的定量评价。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MbpMyelin basic protein ELISA Kit; MBP ELISA Kit
缩写：MBP
Uniprot No.：P02688
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 87-93 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 95-103 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-100 | |||||
回收率：
| The recovery of rat MBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-96 | ||||
| EDTA plasma (n=4) | 95 | 92-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.3332.1732.2532.086101.4021.3771.3901.22350.8680.8880.8780.7112.50.5980.5830.5910.4241.250.4250.4120.4190.2520.6250.2980.3120.3050.1380.3120.2600.2510.2560.08900.1680.1660.167 | ||||||
货期：3-5 working days
×
