首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠巨噬细胞移动抑制因子(MIF)酶联免疫试剂盒检测血清、血浆、组织匀浆样本中的MIF含量。MIF即巨噬细胞移动抑制因子，是一种多功能细胞因子。在炎症、肿瘤等疾病中发挥重要作用。其研究机制主要聚焦在它调控细胞增殖、迁移和炎症反应等方面，通过与受体结合激活下游信号通路，为相关疾病治疗提供了潜在靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于科研场景下对大鼠MIF表达水平的动态监测，例如炎症模型构建、免疫调控机制探索或药物干预效果评估等研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mif ELISA Kit; Macrophage migration inhibitory factor ELISA Kit; MIF ELISA Kit; EC 5.3.2.1 ELISA Kit; Glutathione-binding 13 kDa protein ELISA Kit; L-dopachrome isomerase ELISA Kit; L-dopachrome tautomerase ELISA Kit; EC 5.3.3.12 ELISA Kit; Phenylpyruvate tautomerase ELISA Kit
缩写：MIF
Uniprot No.：P30904
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:5 | Average % | 95 | |
| Range % | 90-100 | ||
| 1:10 | Average % | 98 | |
| Range % | 92-102 | ||
| 1:20 | Average % | 94 | |
| Range % | 89-99 | ||
| 1:40 | Average % | 92 | |
| Range % | 82-96 |
回收率：
| The recovery of rat MIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-94 | ||||
| EDTA plasma (n=4) | 96 | 92-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 40002.7122.6112.6622.507 20002.0541.9532.0041.849 10001.2861.3071.2971.142 5000.7480.7370.7430.588 2500.4780.4670.4730.318 1250.3600.3710.3660.211 62.50.2790.2750.2770.122 00.1550.1540.155 | ||||||
货期：3-5 working days
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