首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠黑色素瘤源性生长调控蛋白(MIA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MIA含量。试剂盒检测范围为4.7 pg/mL-300 pg/mL，适用于科研领域中对黑色素瘤动物模型的研究，如肿瘤微环境分析、转移机制探索、药物干预效果评估等场景，为揭示MIA在肿瘤生物学中的作用及开发相关治疗策略提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mia ELISA Kit; Cdrap ELISA Kit; Mia1 ELISA Kit; Melanoma-derived growth regulatory protein ELISA Kit; Cartilage-derived retinoic acid-sensitive protein ELISA Kit; CD-RAP ELISA Kit; Melanoma inhibitory activity protein ELISA Kit
缩写：MIA
Uniprot No.：Q61865
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：4.7 pg/mL-300 pg/mL
灵敏度：1.17 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MIA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 96 | ||||
| Range % | 82-99 | |||||
| 1:200 | Average % | 101 | ||||
| Range % | 97-105 | |||||
| 1:400 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:800 | Average % | 87 | ||||
| Range % | 85-89 | |||||
回收率：
| The recovery of mouse MIA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 101-107 | ||||
| EDTA plasma (n=4) | 96 | 92-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected3002.5022.5632.5332.4261502.2562.2122.2342.127751.6981.6801.6891.58237.50.8590.8990.8790.77218.750.5140.5360.5250.4189.40.3010.3260.3140.2074.70.1920.1720.1820.07500.1070.1060.107 | ||||||
货期：3-5 working days
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