首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人α葡萄糖苷酶(α-glucosidase)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、尿液样本中的MGAM含量。MGAM即麦芽糖酶-葡糖淀粉酶，在碳水化合物消化中扮演重要角色。研究机制方面，主要聚焦于其在肠道内对淀粉的水解作用，抑制其活性可减少碳水吸收。相关研究有助于开发治疗糖尿病、肥胖症的药物，以调节血糖和体重。试剂盒检测范围为3.12 μU/mL-200 μU/mL，本试剂盒适用于体外科研实验，可帮助研究者探索α - 葡萄糖苷酶在代谢性疾病模型中的表达变化，评估药物或天然产物对酶活性的调控作用，或作为生物标志物用于糖代谢相关病理机制的基础研究。实验操作兼容多种生物样本类型，为体外培养细胞、动物模型或临床前研究提供可靠的定量分析工具，支持从分子机制到功能验证的多层次研究需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：4-alpha-glucosidase ELISA Kit; Alpha-glucosidase ELISA Kit; Glucan 1 ELISA Kit; Glucoamylase ELISA Kit; Maltase-glucoamylase; intestinal ELISA Kit; MGA ELISA Kit; MGA_HUMAN ELISA Kit; MGAM ELISA Kit; MGAML ELISA Kit
缩写：MGAM
Uniprot No.：O43451
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, urine
检测范围：3.12 μU/mL-200 μU/mL
灵敏度：0.78 μU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human α-Glucosidase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:20 | Average % | 95 | |||||
| Range % | 85-103 | ||||||
| 1:40 | Average % | 90 | |||||
| Range % | 85-99 | ||||||
| 1:80 | Average % | 95 | |||||
| Range % | 91-102 | ||||||
| 1:100 | Average % | 89 | |||||
| Range % | 81-97 | ||||||
回收率：
| The recovery of human α-Glucosidase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 85-101 | |||||
| EDTA plasma (n=4) | 93 | 87-99 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| µU/mlOD1OD2AverageCorrected2002.1272.1122.1201.9131001.7461.7571.7521.545501.4421.4171.4301.223251.0691.0281.0490.84212.50.8350.8430.8390.6326.250.5020.5210.5120.3053.120.3470.3540.3510.14400.2050.2090.207 | |||||||
货期：3-5 working days
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