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Human Microfibrillar-associated protein 5(MFAP5) ELISA kit

  • 中文名： 人微纤丝关联蛋白5(MFAP5)酶联免疫试剂盒
货号: IPD-EL013750HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人微纤丝关联蛋白5(MFAP5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MFAP5含量。MFAP5 即微原纤维相关蛋白 5，属于微原纤维相关蛋白家族成员。其与弹性纤维形成、细胞黏附等过程相关。研究机制方面，它在多种疾病如肿瘤、纤维化疾病中异常表达，可能通过影响细胞外基质等发挥作用，是潜在的疾病治疗靶点。试剂盒检测范围为12.5pg/ml- 800pg/ml，适用于心血管疾病机制研究、肿瘤微环境分析、组织再生能力评估等科研领域，适用于体外研究场景，可为探索MFAP5在细胞外基质重塑、疾病生物标志物筛选等方向提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    AAT9 ELISA Kit; MAGP 2 ELISA Kit; MAGP-2 ELISA Kit; MAGP2 ELISA Kit; MFAP 5 ELISA Kit; MFAP-5 ELISA Kit; MFAP5 ELISA Kit; MFAP5_HUMAN ELISA Kit; Microfibril associated glycoprotein 2 ELISA Kit; Microfibril-associated glycoprotein 2 ELISA Kit; microfibrillar associated protein 5 ELISA Kit; Microfibrillar associated protein 5 precursor ELISA Kit; Microfibrillar-associated protein 5 ELISA Kit; MP25 ELISA Kit
  • 缩写：
    MFAP5
  • Uniprot No.：
    Q13361
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    12.5pg/ml - 800pg/ml
  • 灵敏度：
    3.125pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human MFAP5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    97


    Range %

    92-103


    1:2

    Average %

    91


    Range %

    82-97


    1:4

    Average %

    103


    Range %

    99-109


    1:8

    Average %

    87


    Range %

    82-97


  • 回收率：

    The recovery of human MFAP5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    90

    85-99


    EDTA plasma (n=4)

    111

    105-116


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    800

    2.591

    2.612

    2.602

    2.461


    400

    2.247

    2.251

    2.249

    2.108


    200

    1.630

    1.655

    1.643

    1.502


    100

    1.085

    1.074

    1.080

    0.939


    50

    0.654

    0.698

    0.676

    0.535


    25

    0.396

    0.401

    0.399

    0.258


    12.5

    0.263

    0.245

    0.254

    0.113


    0

    0.144

    0.137

    0.141




  • 货期：
    3-5 working days

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