首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人镍纹样蛋白(METRNL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的METRNL含量。METRNL是一种新兴靶点。其它与机体代谢、免疫调节等生理过程相关。研究机制上，它可能通过与特定受体结合，参与信号通路传导，影响细胞的功能和多种生理活动，在糖尿病、炎症等疾病研究中受关注。试剂盒检测范围为0.156 ng/mL-10 ng/mL，探究METRNL在代谢调节中的分子机制、评估肥胖相关模型动物或细胞中蛋白表达变化，以及炎症信号通路与代谢疾病关联性研究，为深入解析METRNL的生物学功能及其在代谢异常中的潜在作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Meteorin like glial cell differentiation regulator ELISA Kit; Meteorin; glial cell differentiation regulator like ELISA Kit; Meteorin-like protein ELISA Kit; METRL_HUMAN ELISA Kit; Metrnl ELISA Kit
缩写：METRNL
Uniprot No.：Q641Q3
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human METRNL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-89 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 86-96 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of human METRNL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 92-103 | ||||
| EDTA plasma (n=4) | 92 | 88-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.6952.5872.6412.47152.0792.1362.1081.9382.51.6821.6041.6431.4731.251.2661.1691.2181.0480.6250.7190.6940.7070.5370.3120.3990.4270.4130.2430.1560.2180.2090.2140.04400.1730.1670.170 | ||||||
货期：3-5 working days
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