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Human Maternal embryonic leucine zipper kinase(MELK) ELISA kit

  • 中文名： 人母体胚胎亮氨酸拉链激酶(MELK)酶联免疫试剂盒
货号: IPD-EL013692HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：人母体胚胎亮氨酸拉链激酶(MELK)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MELK含量。MELK（母体胚胎亮氨酸拉链激酶）是一种在多种恶性肿瘤中高表达的蛋白，作为新型分子靶向治疗靶点，其通过与多种蛋白相互作用调节靶蛋白，在肿瘤增殖、迁移和干细胞特性维持中起关键作用。研究显示，MELK在神经干细胞和脑肿瘤干细胞自我更新中不可或缺，其功能失调与癌细胞逃避免疫监视有关。试剂盒检测范围为0.156 ng/mL-10 ng/mL，本产品适用于基础科研领域，如肿瘤生物学机制研究、干细胞功能探索、抗癌药物开发中的靶点活性评估等场景，为体外实验提供可靠的定量检测工具。实验操作流程标准化，兼容常规酶标仪检测，满足实验室对多种生物样本的分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：AI327312 ELISA Kit; hMELK ELISA Kit; HPK 38 ELISA Kit; hPK38 ELISA Kit; KIAA0175 ELISA Kit; Likely ortholog of maternal embryonic leucine zipper kinase ELISA Kit; Maternal embryonic leucine zipper kinase ELISA Kit; MELK ELISA Kit; MELK_HUMAN ELISA Kit; mKIAA0175 ELISA Kit; MPK38 ELISA Kit; OTTHUMP00000021377 ELISA Kit; OTTHUMP00000046113 ELISA Kit; pEg3 kinase ELISA Kit; Protein kinase Eg3 ELISA Kit; Protein kinase PK38 ELISA Kit; RP23 382O11.1 ELISA Kit; Tyrosine protein kinase MELK ELISA Kit

  • 缩写：MELK

  • Uniprot No.：Q14680

  • 种属：Homo sapiens (Human)

  • 样本类型：serum, plasma, tissue homogenates

  • 检测范围：0.156 ng/mL-10 ng/mL

  • 灵敏度：0.039 ng/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Signal Transduction

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human MELK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)
    1:1Average %94
    Range %90-98
    1:2Average %86
    Range %80-93
    1:4Average %96
    Range %90-101
    1:8Average %100
    Range %96-105

  • 回收率：

    The recovery of human MELK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9488-99
    EDTA plasma (n=4)9589-100














  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected102.7762.8632.8202.67152.1211.9952.0581.9092.51.1421.2411.1921.0431.250.7050.7330.7190.5700.6250.3930.3870.3900.2410.3120.2960.2840.2900.1410.1560.2380.2450.2420.09300.1500.1480.149　






  • 货期：3-5 working days

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