首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠肥大细胞类胰蛋白酶(MCT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MCT含量。MCT（单羧酸转运体）是重要的细胞膜转运蛋白。背景上，它在多种细胞代谢过程中起关键作用。研究机制方面，主要负责转运单羧酸类物质如乳酸等，在能量代谢、细胞内pH调节等过程有重要意义，其功能异常与肿瘤等疾病发展相关。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，广泛应用于科研领域，如肥大细胞活化机制研究、过敏性动物模型评估、炎症相关药物筛选等场景，为探索MCT在免疫调控中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tpsab1 ELISA Kit; Mcpt7 ELISA Kit; Tryptase ELISA Kit; EC 3.4.21.59 ELISA Kit; Mast cell protease 7 ELISA Kit; mMCP-7 ELISA Kit; Tryptase alpha/beta-1 ELISA Kit
缩写：MCT
Uniprot No.：Q02844
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 89-96 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-102 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 93-100 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 88-99 | |||||
回收率：
| The recovery of mouse MCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 93-101 | ||||
| EDTA plasma (n=4) | 91 | 84-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.5622.7562.6592.49710002.1732.2132.1932.0315001.4261.4851.4561.2942500.8010.8450.8230.6611250.5450.5900.5680.40662.50.3090.2930.3010.13931.250.2460.2340.2400.07800.1680.1550.162 | ||||||
货期：3-5 working days
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