首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人中期因子(MK)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MDK含量。MDK即中期因子，是一种分泌性生长因子。其在胚胎发育中表达丰富，出生后表达降低，但在多种肿瘤组织中高表达。研究机制方面，MDK可通过与多种受体结合，激活下游信号通路，促进肿瘤细胞增殖、存活、迁移和血管生成等。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，科研领域可用于探究MK在肿瘤微环境调控、炎症反应及再生医学中的分子机制，或作为评估疾病模型动物及体外实验中MK动态变化的工具，例如通过检测患者血清中MK浓度变化辅助研究其与疾病进展的关联性，或分析肿瘤组织匀浆中MK表达水平以探索靶向治疗策略。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Amphiregulin associated protein ELISA Kit; Amphiregulin-associated protein ELISA Kit; ARAP ELISA Kit; FLJ27379 ELISA Kit; Mdk ELISA Kit; Midgestation and kidney protein ELISA Kit; Midkine ELISA Kit; MK 1 ELISA Kit; MK ELISA Kit; MK_HUMAN ELISA Kit; MK1 ELISA Kit; NEGF 2 ELISA Kit; NEGF2 ELISA Kit; Neurite growth promoting factor 2 ELISA Kit; Neurite outgrowth promoting protein ELISA Kit; Neurite outgrowth-promoting factor 2 ELISA Kit; Neurite outgrowth-promoting protein ELISA Kit; Retinoic acid inducible factor ELISA Kit
缩写：MDK
Uniprot No.：P21741
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 86-97 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 99-107 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 88-98 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 93-103 | |||||
回收率：
| The recovery of human MK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 110 | 100-113 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.6862.6122.6492.532 10002.1772.0892.1332.016 5001.3601.3741.3671.250 2500.7510.7330.7420.625 1250.3590.3450.3520.235 62.50.2960.2870.2920.175 31.250.2010.1910.1960.079 00.1160.1180.117 | ||||||
货期：3-5 working days
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