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产品描述：人细胞外基质磷酸糖蛋白(MEPE)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MEPE含量。MEPE（基质细胞外磷酸糖蛋白）是一种由MEPE基因编码的蛋白质，属于SIBLING蛋白家族，参与骨骼和牙质细胞外基质的构成与骨矿化调节。其研究机制涉及成骨细胞表达、受1,25-(OH)2D3调节，并在牙齿和骨骼发育中发挥重要作用。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，适用于体外研究MEPE在骨代谢疾病模型中的表达调控、矿化相关信号通路机制探索，或评估不同处理条件下（如药物干预、基因修饰）生物样本中MEPE的浓度变化，为骨生物学、矿物质代谢等领域的科研工作提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Matrix extracellular phosphoglycoprotein ELISA Kit; MEPE ELISA Kit; MEPE_HUMAN ELISA Kit; OF45 ELISA Kit; Osteoblast/osteocyte factor 45 ELISA Kit
缩写：MEPE
Uniprot No.：Q9NQ76
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MEPE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| 1:10 | Average % | 98 | ||||
| Range % | 95-102 | |||||
| 1:20 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:40 | Average % | 90 | ||||
| Range % | 85-94 | |||||
回收率：
| The recovery of human MEPE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-102 | ||||
| EDTA plasma (n=4) | 105 | 99-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 10002.6032.7692.6862.516 5001.9672.0752.0211.851 2501.2901.1941.2421.072 1250.6310.6790.6550.485 62.50.4760.5060.4910.321 31.20.3410.3570.3490.179 15.60.2540.2460.2500.080 00.1700.1690.170 | ||||||
货期：3-5 working days
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