首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠乳凝集素/乳脂肪球EGF因子蛋白8(MFGE8)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MFGE8含量。MFGE8，也称为乳脂肪球表皮生长因子8，是一种分泌蛋白，主要在椎骨干细胞中表达。研究发现，MFGE8能吸引肿瘤细胞并促进其生长，是肿瘤骨转移的关键因素。研究机制表明，MFGE8通过诱导癌细胞迁移和促进其生长，在肿瘤转移中发挥重要作用。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于科研领域中对MFGE8表达水平的研究，如炎症性疾病模型、组织再生实验或代谢相关疾病的分子机制探索，为评估生物标志物动态变化提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mfge8Lactadherin ELISA Kit; MFGM ELISA Kit; Milk fat globule-EGF factor 8 ELISA Kit; MFG-E8 ELISA Kit; SED1 ELISA Kit; Sperm surface protein SP47 ELISA Kit; MP47 ELISA Kit
缩写：MFGE8
Uniprot No.：P21956
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MFGE8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 102 | |||||
| Range % | 94-107 | ||||||
| 1:2 | Average % | 104 | |||||
| Range % | 98-108 | ||||||
| 1:4 | Average % | 101 | |||||
| Range % | 95-106 | ||||||
| 1:8 | Average % | 107 | |||||
| Range % | 100-111 | ||||||
回收率：
| The recovery of mouse MFGE8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 86-97 | |||||
| EDTA plasma (n=4) | 99 | 90-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 20002.5732.4882.5312.335 10001.8451.8981.8721.676 5001.1221.1561.1390.943 2500.6490.6310.6400.444 1250.4670.4530.4600.264 62.50.3440.3370.3410.145 31.250.2890.2820.2860.090 00.1930.1990.196 | |||||||
货期：3-5 working days
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