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产品描述：人乳脂肪球EGF因子蛋白8(MFGE8)酶联免疫试剂盒双抗夹心法ELISA试剂盒，定量检测血清、组织培养上清液、尿液、脑脊液（CSF）样本中的MFGE8含量。人乳脂肪球EGF因子蛋白8（MFGE8）是一种分泌型糖蛋白，广泛表达于多种组织细胞中。研究发现，MFGE8在心脏重构和心力衰竭的诊断及治疗中具有重要作用，其表达水平与心脏重构和心力衰竭严重程度密切相关，且具有抑制心肌肥厚、心肌纤维化，改善心功能的作用。试剂盒检测范围为781.25 pg/mL-50000 pg/mL，科研人员可将其用于探索MFGE8在炎症性疾病、代谢紊乱、神经退行性疾病及癌症等领域的生物学功能，例如通过检测细胞上清液中MFGE8的动态变化研究其分泌机制，或分析临床前模型中体液样本的蛋白表达差异；为体外实验提供可靠工具，助力基础研究与转化医学中的分子机制解析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BA46 ELISA Kit; Breast epithelial antigen BA46 ELISA Kit; EDIL1 ELISA Kit; HMFG ELISA Kit; hP47 ELISA Kit; HsT19888 ELISA Kit; Lactadherin ELISA Kit; Medin ELISA Kit; MFG-E8 ELISA Kit; MFGE8 ELISA Kit; MFGM ELISA Kit; MFGM_HUMAN ELISA Kit; Milk fat globule EGF factor 8 ELISA Kit; Milk fat globule EGF factor 8 protein ELISA Kit; Milk fat globule-EGF factor 8 ELISA Kit; O acetyl disialoganglioside synthase ELISA Kit; OAcGD3S ELISA Kit; SED1 ELISA Kit; SPAG10 ELISA Kit; Sperm associated antigen 10 ELISA Kit; Sperm surface protein hP47 ELISA Kit
缩写：MFGE8
Uniprot No.：Q08431
种属：Homo sapiens (Human)
样本类型：serum, cell culture supernates, urine, cerebrospinal fluid (CSF)
检测范围：781.25 pg/mL-50000 pg/mL
灵敏度：195.31 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MFGE8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 85-100 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-96 | |||||
| 1:8 | Average % | 85 | ||||
| Range % | 80-93 | |||||
回收率：
| The recovery of human MFGE8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 85-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected500002.4372.4522.4452.264250001.8851.8761.8811.700125001.3041.3241.3141.13362500.8210.8540.8380.65731250.5950.5840.5900.4091562.50.4140.4360.4250.244781.250.2890.2790.2840.10300.1740.1870.181 | ||||||
货期：3-5 working days
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