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产品描述：小鼠基质γ羧基谷氨酸蛋白(MGP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MGP含量。小鼠基质γ羧基谷氨酸蛋白（MGP）是一种维生素K依赖性蛋白，主要存在于骨骼、软骨、心脏、肾脏和肺等器官中，具有抑制钙离子在软组织异常沉积的作用，与多种疾病如骨质疏松、动脉硬化等密切相关。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，科研人员可通过探究MGP在血管钙化模型、骨代谢疾病动物实验中的动态变化，或结合细胞实验研究其在矿化相关信号通路中的作用机制，为心血管及骨骼系统疾病的分子机制研究提供可靠工具。该产品严格遵循科研用途设计，适用于基础研究中的蛋白表达定量及病理生理机制分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mgp ELISA Kit; Mglap ELISA Kit; Matrix Gla protein ELISA Kit; MGP ELISA Kit
缩写：MGP
Uniprot No.：P19788
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 96 | ||||
| Range % | 93-99 | |||||
| 1:200 | Average % | 87 | ||||
| Range % | 85-89 | |||||
| 1:400 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:800 | Average % | 104 | ||||
| Range % | 102-106 | |||||
回收率：
| The recovery of mouse MGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 86 | 83-89 | ||||
| EDTA plasma (n=4) | 87 | 85-89 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.4322.4682.4502.33710002.1692.1502.1602.0475001.7041.7351.7201.6072501.1151.1361.1261.0131250.5680.5980.5830.47062.50.3440.3680.3560.24331.250.2260.2360.2310.11800.1140.1120.113 | ||||||
货期：3-5 working days
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