首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人单甘油脂酶(MGLL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MGLL含量。MGLL是重要靶点。它在体内参与内源性大麻素系统的代谢过程。研究发现其可能调节神经信号传导、炎症反应等生理机制。对其深入研究有助于了解相关疾病病理，有望为开发神经系统、炎症性疾病等药物提供新方向。试剂盒检测范围为39.06 pg/mL-2500 pg/mL，适用于基础科研中探究MGLL在代谢调控网络中的作用、评估疾病模型（如非酒精性脂肪肝、2型糖尿病）中酶活性变化，或用于药物干预实验的分子标记物检测，为脂代谢通路研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：EC 3.1.1.23 ELISA Kit; HU K5 ELISA Kit; HU-K5 ELISA Kit; HUK5 ELISA Kit; Lysophospholipase homolog ELISA Kit; Lysophospholipase like ELISA Kit; Lysophospholipase-like ELISA Kit; MAGL ELISA Kit; MGL ELISA Kit; MGLL ELISA Kit; MGLL_HUMAN ELISA Kit; Monoacylglycerol lipase ELISA Kit; Monoglyceride lipase ELISA Kit
缩写：MGLL
Uniprot No.：Q99685
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：39.06 pg/mL-2500 pg/mL
灵敏度：9.76 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MGLL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 101-107 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 93-104 | |||||
回收率：
| The recovery of human MGLL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 82-91 | ||||
| EDTA plasma (n=4) | 94 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected25002.5242.5372.5312.37012501.6611.6871.6741.5136251.0761.0821.0790.918312.50.7340.7040.7190.558156.250.5110.5040.5080.34778.130.3750.3920.3840.22339.060.3020.2980.3000.13900.1650.1570.161 | ||||||
货期：3-5 working days
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