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产品描述：兔子巨噬细胞炎性蛋白1α(MIP-1α/CCL3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MIP1α/CCL3含量。MIP1α/CCL3，即巨噬细胞炎症蛋白1-a/CCL3，是CC亚家族的趋化因子，主要由单核细胞、巨噬细胞等免疫细胞产生。它通过结合细胞表面受体CCR1、CCR3和CCR5，诱导白细胞趋化，促进T细胞进入炎症区域，并在肉芽肿、哮喘等自身免疫性疾病中发挥关键作用。试剂盒检测范围为7.8 pg/mL-500 pg/mL，科研人员可将其应用于炎症机制研究、疾病模型中的趋化因子动态监测、免疫调节药物筛选等方向，为探索MIP - 1α在先天免疫应答、感染性疾病或自身免疫病中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MIP-1 alpha,Macrophage Inflammatory Protein-1 alpha
缩写：MIP1α/CCL3
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MIP-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 105 | |||||
| Range % | 93-109 | ||||||
| 1:2 | Average % | 110 | |||||
| Range % | 97-114 | ||||||
| 1:4 | Average % | 88 | |||||
| Range % | 83-93 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 85-98 | ||||||
回收率：
| The recovery of rabbit MIP-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 105 | 95-109 | |||||
| EDTA plasma (n=4) | 101 | 91-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 5002.8032.7362.7702.629 2502.3622.4552.4092.268 1251.7431.8621.8031.662 62.51.1231.1511.1370.996 31.250.7300.7440.7370.596 15.60.5500.5370.5440.403 7.80.3060.3120.3090.168 00.1390.1430.141 | |||||||
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