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产品描述：犬胃动素(MLN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MLN含量。试剂盒检测范围为125 pg/mL-8000 pg/mL，该产品适用于科研领域，例如在动物营养学实验中评估饲料成分对胃肠激素分泌的影响，或在基础医学研究中探索胃肠功能紊乱的分子机制，为犬类消化生理及病理研究提供定量分析支持。研究犬消化系统疾病模型、胃肠动力调节机制或相关药物干预实验。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MLN ELISA Kit; Motilin ELISA Kit
缩写：MLN
Uniprot No.：P19863
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog MLN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of dog MLN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 80002.1882.0962.1421.967 40001.4871.4061.4471.272 20000.9620.9870.9750.800 10000.6260.6070.6170.442 5000.4340.4680.4510.276 2500.3610.3350.3480.173 1250.2920.2810.2870.112 00.1710.1780.175 | ||||||
货期：3-5 working days
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