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产品描述：大鼠脑啡肽酶(MME)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MME含量。MME即膜金属肽酶，它在人体生理过程如血压调节、神经信号传导等方面有重要作用。研究中其机制主要聚焦于作为药物靶点，通过调节其活性来干预特定疾病进展，如在心血管疾病、神经系统疾病等领域是潜在治疗方向。试剂盒检测范围为47 pg/mL-3000 pg/mL，适用于基础科研中探究神经系统功能、药物干预对MME活性调控、疾病模型机制分析等方向，为神经生物学、药理学及病理学研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MmeNeprilysin ELISA Kit; EC 3.4.24.11 ELISA Kit; Atriopeptidase ELISA Kit; Enkephalinase ELISA Kit; Neutral endopeptidase 24.11 ELISA Kit; NEP ELISA Kit; Neutral endopeptidase ELISA Kit; Skin fibroblast elastase ELISA Kit; SFE ELISA Kit; CD antigen CD10 ELISA Kit
缩写：MME
Uniprot No.：P07861
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MME in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 89-96 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 91-103 | ||||||
| 1:4 | Average % | 98 | |||||
| Range % | 93-101 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 90-99 | ||||||
回收率：
| The recovery of rat MME spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 89-96 | |||||
| EDTA plasma (n=4) | 98 | 93-103 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected30002.2562.3452.3012.10415001.6341.5361.5851.3887501.1281.2141.1710.9743750.7740.7870.7810.584187.50.4790.4820.4810.284940.3570.3650.3610.164470.2680.2690.2690.07200.1950.1990.197 | |||||||
货期：3-5 working days
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