关键信息
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基因名
MAF1
- 应用
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别名
Homolog of yeast MAF1; maf1; MAF1 homolog (S. cerevisiae); MAF1 homolog; MAF1_HUMAN; MGC20332; MGC31779; MGC39758; Repressor of RNA polymerase III transcription MAF1 homolog
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种属
Human
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表达系统
E. coli
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标签
N- His-SUMO
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9H063
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表达区间
1-256aa
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分子量
44.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
MAF1 (Multiply Attenuated Factor 1) is a critical regulatory protein that plays a significant role in the control of tRNA gene transcription in response to cellular energy levels. The research on MAF1 has gained momentum due to its involvement in various cellular processes and its potential implications in diseases, including cancer and metabolic disorders. MAF1’s primary function is to inhibit RNA polymerase III activity when cellular stress conditions, such as nutrient deprivation, occur. This regulation is essential for maintaining cellular homeostasis and ensuring that protein synthesis is prioritized under optimal conditions. Studies have demonstrated that post-translational modifications, such as phosphorylation, can modulate MAF1's activity, highlighting its multifunctional nature. Additionally, MAF1 has been identified as a potential tumor suppressor, with altered expression levels linked to tumorigenesis. The exploration of MAF1’s structure, function, and regulatory mechanisms has sparked interest in understanding its role in both normal physiology and disease states. Researchers are utilizing recombinant MAF1 proteins to dissect its interactions with other cellular components, aiming to elucidate its comprehensive role in gene expression and metabolism. This investigation could open new avenues for therapeutic strategies targeting MAF1 in diseases characterized by dysregulated transcriptional control. As the field advances, the integration of structural biology, biochemistry, and molecular biology continues to shed light on the myriad functions of MAF1, positioning it as a crucial player in the intricate network of cellular regulation. Understanding the nuances of MAF1’s function may lead to significant breakthroughs in the treatment of conditions linked to disrupted cellular energy sensing and transcriptional regulation.
