首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠基质金属蛋白酶3(MMP-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MMP3含量。MMP3，即基质金属蛋白酶3，是一种在多种生理和病理过程中发挥关键作用的酶类分子。它在炎症性疾病、肿瘤、动脉粥样硬化和神经系统疾病中扮演重要角色，能够降解多种基质成分，并激活其他MMPs，参与组织重塑和疾病进程。研究显示，MMP3的基因多态性与某些疾病的遗传易感性相关，如食管鳞癌。试剂盒检测范围为1.56 pg/mL-100 pg/mL，本产品可广泛应用于基础医学研究领域，如关节炎动物模型中的基质代谢研究、药物干预下MMP - 3表达水平动态监测，或肿瘤微环境中蛋白酶活性评估等实验场景，为组织重塑机制探索及病理模型构建提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mmp3 ELISA Kit; Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit; PTR1 protein ELISA Kit; Transin-1 ELISA Kit
缩写：MMP3
Uniprot No.：P03957
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 90 | ||||
| Range % | 87-93 | |||||
| 1:200 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 88-96 | |||||
回收率：
| The recovery of rat MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 81-89 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.5692.5472.5582.429502.2362.2132.2252.096251.8681.8291.8491.72012.51.3671.3871.3771.2486.250.8400.8890.8650.7363.130.3770.3870.3820.2531.560.2470.2590.2530.12400.1280.1300.129 | ||||||
货期：3-5 working days
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