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产品描述：犬基质金属蛋白酶3(MMP-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液、组织匀浆样本中的MMP3含量。MMP3 即基质金属蛋白酶 3，它在组织重塑、炎症等生理病理过程中扮演重要角色。研究发现其异常表达与多种疾病如关节炎、肿瘤发展相关。机制上，它可降解细胞外基质成分，影响细胞微环境，促进细胞迁移和肿瘤转移，是极具潜力的治疗靶点。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于科研场景中犬类生物标志物研究，例如探索炎症相关疾病的分子机制、评估药物对组织修复的影响或建立特定病理模型。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MMP3Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit
缩写：MMP3
Uniprot No.：Q6Y4Q5
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, cell culture supernates, urine, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-96 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 92-105 | |||||
回收率：
| The recovery of dog MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-99 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.4102.5452.4782.284501.9601.9821.9711.777251.5951.5631.5791.38512.51.1771.2011.1890.9956.250.7860.7890.7880.5943.120.4580.4620.4600.2661.560.2780.2620.2700.07600.1930.1950.194 | ||||||
货期：3-5 working days
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