首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：猪基质金属蛋白酶2/明胶酶A(MMP-2/Gelatinase A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MMP2/Gelatinase A含量。MMP2/Gelatinase A即基质金属蛋白酶2，在细胞外基质代谢、组织重塑等生理过程中发挥重要作用。异常表达与肿瘤侵袭转移、心血管疾病和关节炎等多种疾病相关。研究多聚焦其酶活性调控机制，寻找抑制其活性的靶点，为疾病治疗提供新思路。试剂盒检测范围为47 ng/mL-3000 ng/mL，适用于科研领域探究猪模型中的组织再生机制、疾病相关分子通路及药物干预效果评估等方向，为深入解析MMP - 2在生理病理中的功能提供高效检测方案本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MMP2/Gelatinase A
Uniprot No.：F1RF11
种属：Sus scrofa (Pig)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：47 ng/mL-3000 ng/mL
灵敏度：11.75 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 92-97 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 99-105 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-99 | |||||
回收率：
| The recovery of pig MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-97 | ||||
| EDTA plasma (n=4) | 90 | 86-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected30002.4372.5062.4722.37915001.9881.9661.9771.8847501.5651.6461.6061.5133751.1631.2591.2111.118187.50.7740.7640.7690.676940.3520.3670.3600.267470.2220.2140.2180.12500.0960.0890.093 | ||||||
货期：3-5 working days
×
