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产品描述：犬基质金属蛋白酶13(MMP-13)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MMP-13含量。MMP-13即基质金属蛋白酶13，在人体组织重塑、炎症反应等生理过程中起重要作用。异常表达与多种病理情况相关，如关节炎、癌症等。研究主要围绕其酶活性调控机制，旨在开发针对此靶点的药物，以干预相关疾病进展。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于科研领域对犬类骨关节疾病模型、软骨退行性病变机制以及抗炎药物研发等研究方向的样本检测需求；满足实验室对犬MMP - 13蛋白表达水平的高通量检测及动态监测要求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MMP-13
Uniprot No.：F1PVS9
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 88-99 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 95-106 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 84-92 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 85-98 | |||||
回收率：
| The recovery of canine MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-95 | ||||
| EDTA plasma (n=4) | 88 | 85-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected103.1603.2553.2083.10752.6142.7122.6632.5622.51.7841.9321.8581.7571.251.0261.0301.0280.9270.6250.4780.4870.4830.3820.3120.2640.2560.2600.1590.1560.1560.1660.1610.06000.1050.0970.101 | ||||||
货期：3-5 working days
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