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产品描述：小鼠巨噬细胞金属弹力酶(MMP12)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、cellculture匀浆样本中的MMP12含量。MMP12即基质金属蛋白酶12，在多种生理病理过程中发挥作用。其可降解细胞外基质成分，与组织重塑等密切相关。在研究中，它常作为靶点探索疾病机制，比如与肺部炎症、肿瘤侵袭转移等的关联，有望为相关疾病治疗提供新策略。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于体外研究小鼠模型中MMP12的表达调控及其在炎症微环境、纤维化疾病或肿瘤微环境中的作用机制分析，支持科研人员对生物样本中MMP12的精准定量分析，为探索相关疾病的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mmp12 ELISA Kit; Mme ELISA Kit; Mmel ELISA Kit; Macrophage metalloelastase ELISA Kit; MME ELISA Kit; EC 3.4.24.65 ELISA Kit; Matrix metalloproteinase-12 ELISA Kit; MMP-12 ELISA Kit
缩写：MMP12
Uniprot No.：P34960
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell culture homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 87 | ||||
| Range % | 83-96 | |||||
| 1:200 | Average % | 98 | ||||
| Range % | 92-105 | |||||
| 1:400 | Average % | 102 | ||||
| Range % | 98-108 | |||||
| 1:800 | Average % | 94 | ||||
| Range % | 89-98 | |||||
回收率：
| The recovery of mouse MMP12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 92-104 | ||||
| EDTA plasma (n=4) | 91 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.8012.7222.7622.6082502.1102.2142.1622.0081251.4271.4481.4381.28462.50.8560.8670.8620.70831.20.5230.5120.5180.36415.60.3510.3410.3460.1927.80.2640.2600.2620.10800.1540.1530.154 | ||||||
货期：3-5 working days
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