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产品描述：人基质金属蛋白酶10(MMP-10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MMP10含量。MMP10 是重要靶点。它属于基质金属蛋白酶家族，在细胞外基质代谢、组织重塑等过程起关键作用。研究发现其异常表达与肿瘤侵袭转移、炎症等疾病密切相关，目前围绕其开展的研究致力于阐明机制以开发针对性治疗手段。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于基础科研中探索MMP-10在疾病模型中的表达调控机制、药物干预效果评估或病理微环境动态变化研究。满足细胞实验、动物模型及离体样本的定量分析需求，为研究细胞外基质代谢相关通路提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Matrix metallopeptidase 10 (stromelysin 2) ELISA Kit; Matrix metalloprotease 10 ELISA Kit; Matrix metalloproteinase 10 (stromelysin 2) ELISA Kit; Matrix metalloproteinase 10 ELISA Kit; Matrix metalloproteinase-10 ELISA Kit; MMP 10 ELISA Kit; MMP-10 ELISA Kit; Mmp10 ELISA Kit; MMP10_HUMAN ELISA Kit; SL 2 ELISA Kit; SL-2 ELISA Kit; SL2 ELISA Kit; STMY 2 ELISA Kit; STMY2 ELISA Kit; Stromelysin 2 ELISA Kit; Stromelysin II ELISA Kit; Stromelysin-2 ELISA Kit; Stromelysin2 ELISA Kit; StromelysinII ELISA Kit; Transin 2 ELISA Kit; Transin-2 ELISA Kit; Transin2 ELISA Kit
缩写：MMP10
Uniprot No.：P09238
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 96 | |||||
| Range % | 90-102 | ||||||
| 1:2 | Average % | 107 | |||||
| Range % | 102-110 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 90-101 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 86-96 | ||||||
回收率：
| The recovery of human MMP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 103 | 99-105 | |||||
| EDTA plasma (n=4) | 94 | 90-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected50001.8991.8771.8881.77925001.3541.3661.3601.25112500.9240.9660.9450.8366250.6220.6540.6380.5293120.4330.4550.4440.3351560.2700.2780.2740.165780.2380.2330.2360.12700.1120.1050.109 . | |||||||
货期：3-5 working days
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