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产品描述：兔基质金属蛋白酶1(MMP-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MMP1含量。MMP1 即基质金属蛋白酶 1，在细胞外基质降解中发挥关键作用。异常表达与肿瘤侵袭转移、关节炎等疾病密切相关。其研究聚焦于解析活性调控机制和与疾病信号通路的关联，旨在开发针对性药物以干预相关病理进程。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于基础医学研究领域，如探究心血管疾病、关节炎或皮肤纤维化等疾病模型中MMP-1的调控机制，也可用于药物开发过程中对MMP-1抑制剂的体外效果评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MMP1Interstitial collagenase ELISA Kit; EC 3.4.24.7 ELISA Kit; Matrix metalloproteinase-1 ELISA Kit; MMP-1 ELISA Kit
缩写：MMP1
Uniprot No.：P13943
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-100 | |||||
| 1:2 | Average % | 104 | ||||
| Range % | 97-109 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 82-92 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 91-102 | |||||
回收率：
| The recovery of rabbit MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 82-92 | ||||
| EDTA plasma (n=4) | 102 | 92-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.5902.5452.5682.46651.9631.9221.9431.8412.51.4351.4001.4181.3161.250.8410.8180.8300.7280.6250.4480.4270.4380.3360.3120.3280.3170.3230.2210.1560.2480.2420.2450.14300.1010.1030.102 | ||||||
货期：3-5 working days
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