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产品描述：犬基质金属蛋白酶1(MMP-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MMP-1含量。MMP-1，又称间质胶原酶和成纤维细胞胶原酶，是基质金属蛋白酶家族的一员，主要参与细胞外基质的降解。其在正常生理过程中如胚胎发育和组织重塑中起作用，同时在疾病过程中如癌症和骨关节炎中，MMP-1的表达和活性增加，导致细胞外基质破坏，促进疾病进展。试剂盒检测范围为0.18 ng/mL-12 ng/mL，适用于科研场景中探究犬类疾病模型（如关节炎、皮肤损伤修复或肿瘤微环境）中MMP-1的动态变化。可为分子机制研究、药物开发或生物标志物筛选提供定量数据支持，满足科研工作者对犬类MMP-1活性监测的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MMP-1
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：0.18 ng/mL-12 ng/mL
灵敏度：0.04 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 85-102 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-99 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 83-98 | |||||
回收率：
| The recovery of canine MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 82-98 | ||||
| EDTA plasma (n=4) | 97 | 89-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected122.3092.3262.3182.11461.7541.6991.7271.52331.3011.3381.3201.1161.50.9310.9200.9260.7220.750.5730.5850.5790.3750.370.4120.4070.4100.2060.180.2990.3030.3010.09700.2010.2060.204 | ||||||
货期：3-5 working days
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