首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠基质金属蛋白酶1(MMP-1)酶联免疫试剂盒检测血清、血浆、组织培养上清液、组织匀浆样本中的MMP-1含量。MMP-1，也称为间质胶原酶和成纤维细胞胶原酶，是基质金属蛋白酶家族的一员。它参与细胞外基质的降解，在正常生理过程和疾病过程中发挥作用。MMP-1通过降解胶原蛋白和明胶等成分，影响细胞微环境，与肿瘤侵袭、转移和骨关节炎等疾病相关。研究MMP-1有助于开发针对相关疾病的治疗策略。试剂盒检测范围为47 pg/mL-3000 pg/mL，适用于体外实验研究，可支持细胞功能探索、疾病模型建立及药物作用机制分析等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mmp1a ELISA Kit; McolAInterstitial collagenase A ELISA Kit; EC 3.4.24.7 ELISA Kit; Matrix metalloproteinase-1a ELISA Kit; MMP-1a ELISA Kit; Mcol-A ELISA Kit
缩写：MMP-1
Uniprot No.：Q9EPL5
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:20 | Average % | 91 | |
| Range % | 86-95 | ||
| 1:40 | Average % | 100 | |
| Range % | 97-103 | ||
| 1:80 | Average % | 91 | |
| Range % | 87-95 | ||
| 1:160 | Average % | 97 | |
| Range % | 93-100 |
回收率：
| The recovery of mouse MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 96 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 30002.4822.5102.4962.317 15001.8551.7861.8211.642 7501.2461.2321.2391.060 3750.7480.7740.7610.582 187.50.4950.4560.4760.297 93.750.3220.3390.3310.152 46.880.2530.2420.2480.069 00.1800.1780.179 | ||||||
货期：3-5 working days
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