首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠基质金属蛋白酶10(MMP-10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MMP10含量。MMP10 即基质金属蛋白酶 10，在细胞外基质降解与重塑中发挥关键作用。其高表达与肿瘤的侵袭、转移及血管生成相关。研究机制聚焦于它对基质成分的酶解作用，以及与细胞信号通路的交互，以探索其在疾病诊疗中的应用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，能够支持研究者在小鼠模型的组织纤维化、肿瘤侵袭转移或炎症性疾病等机制研究中，动态监测MMP - 10的表达变化，为探索其在病理生理过程中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mmp10 ELISA Kit; Stromelysin-2 ELISA Kit; SL-2 ELISA Kit; EC 3.4.24.22 ELISA Kit; Matrix metalloproteinase-10 ELISA Kit; MMP-10 ELISA Kit; Transin-2 ELISA Kit
缩写：MMP10
Uniprot No.：O55123
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 105 | |||||
| Range % | 101-114 | ||||||
| 1:2 | Average % | 91 | |||||
| Range % | 86-95 | ||||||
| 1:4 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
| 1:8 | Average % | 87 | |||||
| Range % | 82-93 | ||||||
回收率：
| The recovery of mouse MMP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 88-96 | |||||
| EDTA plasma (n=4) | 84 | 80-88 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected10002.5172.4012.4592.2465001.7911.7361.7641.5512501.1051.0581.0820.8691250.6830.6620.6730.46062.50.4120.4090.4110.19831.250.3290.3400.3350.12215.60.2680.2730.2710.05800.2100.2150.213 | |||||||
货期：3-5 working days
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