首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠基质金属蛋白酶10(MMP-10)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MMP10含量。MMP10 即基质金属蛋白酶 10，在多种生物学过程如组织重塑、细胞迁移等中发挥作用。其异常表达与肿瘤生长、侵袭和转移等密切相关。研究机制主要聚焦于调控细胞外基质降解，以及与其他信号通路相互作用等方面，是颇具潜力的治疗靶点。试剂盒检测范围为80 pg/mL-2000 pg/mL，该产品可广泛应用于炎症性疾病机制探究、肿瘤转移模型分析，以及心血管疾病或肝纤维化等科研领域的基础研究，为探索MMP - 10的生物学功能提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mmp10 ELISA Kit; Stromelysin-2 ELISA Kit; SL-2 ELISA Kit; EC 3.4.24.22 ELISA Kit; Matrix metalloproteinase-10 ELISA Kit; MMP-10 ELISA Kit; Transformation-associated protein 34A ELISA Kit; Transin-2 ELISA Kit
缩写：MMP10
Uniprot No.：P07152
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：80 pg/mL-2000 pg/mL
灵敏度：50 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<15% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 85-99 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 90-103 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 87-105 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 87-99 | ||||||
回收率：
| The recovery of rat MMP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 96 | 88-100 | |||||
| EDTA plasma (n=4) | 95 | 89-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2Average20000.1720.1750.1748000.3760.3790.3784800.5540.5620.5582000.8820.8970.890801.4931.4751.48402.4042.4522.428 | |||||||
货期：3-5 working days
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