首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠基质金属蛋白酶13(MMP-13)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MMP13含量。MMP13 即基质金属蛋白酶 13，在人体中参与细胞外基质的降解和重塑。其异常表达与关节炎、肿瘤等疾病发展相关。研究聚焦于其在疾病进程中作用机制，期望通过抑制 MMP13 活性，为相关疾病治疗提供新思路。试剂盒检测范围为93.75 pg/mL-6000 pg/mL，适用于基础科研中关节炎模型评估、肿瘤微环境研究或骨代谢相关实验的数据获取，为体外研究MMP - 13的表达调控及功能机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mmp13 ELISA Kit; Collagenase 3 ELISA Kit; EC 3.4.24.- ELISA Kit; Matrix metalloproteinase-13 ELISA Kit; MMP-13 ELISA Kit
缩写：MMP13
Uniprot No.：P33435
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：93.75 pg/mL-6000 pg/mL
灵敏度：23.438 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 85-100 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 82-98 | |||||
回收率：
| The recovery of mouse MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 81-98 | ||||
| EDTA plasma (n=4) | 94 | 92-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected60002.7202.6422.6812.45330002.4162.3812.3992.17115002.1122.0432.0781.8507501.7321.6901.7111.4833751.3371.2441.2911.063187.50.9140.8760.8950.66793.750.5340.4920.5130.28500.2370.2190.228 | ||||||
货期：3-5 working days
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